Preparation and Evaluation of Master Seed for infectious bronchitis vaccine from local variant isolate
نویسندگان
چکیده
Preparation and Evaluation of Master seed prepared from Local variant infectious bronchitis virus (IBV) isolated from different poultry farms in Egypt and identified as IS/1494/06 nephropathogenic strain are very important point for vaccine production for controlling of infectious brobchitis(IB) disease in Egypt. Master seed of the virus (MSV) contained 10 EID50 per dose per bird free from bacterial; fungal; mycoplasma ; extraneous viral contamination ; Safe and potent. Polymerase chain reaction (PCR) genotyping methods used for determining the relation between MSV and other IB variant strains (IB-88 and IB 4-91). The molecular basis of antigenic variation has been investigated in showed a distinct dissimilarity with either CR-88 ( 27.83%) or IB4-91(9.3%) . [Susan, S. El-Mahdy; Y. A., Soliman and M. M. El-Hady Preparation and Evaluation of Master Seed for infectious bronchitis vaccine from local variant isolate] Nature and Science 2011; 9(10):145-151]. (ISSN: 1545-0740). http://www.sciencepub.net. 19
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